烟草NtTGA4a 转录因子在烟草抗烟草黑胫病中的功能研究
首发时间:2023-05-12
摘要:由寄生疫霉(Phytophthora parasitica)引发的烟草黑胫病是烟草种植领域的难题,每年给我国烟草产业带来巨大的经济损失。鉴定烟草黑胫病抗病基因,提高烟草对烟草疫霉的抗性能力,对于培育烟草黑胫病抗病新品种,防治烟草黑胫病害具有重要意义,是解决烟草黑胫病病害的根本途径。但是当前对烟草黑胫病抗病机理研究较少,亟待深入解析烟草响应烟草黑胫病的分子机理,为培育对烟草黑胫病有优良抗性的作物品种提供理论基础。TGAs(TGACG motif-binding factor)转录因子属于bZIP转录因子家族D亚族,该亚族成员主要参与植物防御病害及生长发育过程。在植物免疫应答中,TGAs转录因子通过调节植物免疫相关靶基因的转录从而帮助植物抵御病害。本文初步研究发现烟草NtTGA4a在调控烟草免疫反应中具有重要功能,该研究将为植物寄生疫霉病害的防治提供重要的理论基础,筛选出可用于农作物基因工程改良的靶标基因,为培育烟草疫霉抗病品种提供新的分子标记,具有重要的理论意义和应用价值。
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Function of NtTGA4a in tobacco against Phytophthora nicotiana
Abstract:Tobacco black shank caused by Phytophthora parasitica is a very big problem in tobacco planting, which leads great economic loss to tobacco industry every year in China. It is the fundamental way to solve tobacco black shank by breeding and cultivating phytophthora parasitica resistance varieties. However, little is known about the pathogenicity of Phytophthora parasitica and the mechanism of tobacco resistance to Phytophthora nicotiana.Revealing the underlying molecular mechanism of tobacco resistance to Phytophthora nicotiana may provide an important theoretical basis for breeding tobacco black shank resistance varieties. TGA (TGACG motif-binding factor) transcription factor belongs to subfamily D of bZIP transcription factor family. Members of this subfamily are involved defense response and growth and development in plant. In plant immune response, TGAs transcription factors help plants to resist diseases by regulating the expression of plant immune-related target genes. This preliminary study found that tobacco NtTGA4a have important roles in tobacco response to Phytophthora parasitica, and this study provided an important theoretical basis for controling tobacco black shank, and provided new molecular markers for breeding tobacco black shank resistant varieties.
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